Proton Transfer via Protein Bound Water Molecules
نویسندگان
چکیده
منابع مشابه
How does a membrane protein achieve a vectorial proton transfer via water molecules?
We present a detailed mechanism for the proton transfer from a protein-bound protonated water cluster to the bulk water directed by protein side chains in the membrane protein bacteriorhodopsin. We use a combined approach of time-resolved Fourier transform infrared spectroscopy, molecular dynamics simulations, and X-ray structure analysis to elucidate the functional role of a hydrogen bond betw...
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High-resolution protein ground-state structures of proton pumps and channels have revealed internal protein-bound water molecules. Their possible active involvement in protein function has recently come into focus. An illustration of the formation of a protonated protein-bound water cluster that is actively involved in proton transfer was described for the membrane protein bacteriorhodopsin (bR...
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The nicotinamide nucleotide transhydrogenase (TH) is an integral membrane enzyme that uses the proton-motive force to drive hydride transfer from NADH to NADP+ in bacteria and eukaryotes. Here we solved a 2.2-Å crystal structure of the TH transmembrane domain (Thermus thermophilus) at pH 6.5. This structure exhibits conformational changes of helix positions from a previous structure solved at p...
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Molecular oxygen acts as the terminal electron sink in the respiratory chains of aerobic organisms. Cytochrome c oxidase in the inner membrane of mitochondria and the plasma membrane of bacteria catalyzes the reduction of oxygen to water, and couples the free energy of the reaction to proton pumping across the membrane. The proton-pumping activity contributes to the proton electrochemical gradi...
متن کاملProton Transfer Reactions and Kinetics in Water
Introduction and Survey A. Molecular Nature of Pure Water B. Dissociation and Electrical Conduction Measurements c. Isotope Effects D. Chemical Reactions Involving Proton Transfer Potential Energy Surfaces A. Bond Breakage B. Hydrated Proton C. Hydrated Hydroxide General Theory of Association-Dissociation Reactions A. Definition of Chemical Species B. Molecular Distribution Functions c. Linear ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.2073